2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt - 1 g

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    CAS Number: 30931-67-0

    Molecular Weight: 548.68

    Molecular Formula: C18H24N6O6S4

    Appearance: Powder

    Physical State: Solid

    Solubility: Soluble in Water: 50 mg/mL

    pH: 7

    Storage: Store at -20° C


    2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). 


    References:

    1. Bubble-free oxygenation of a bi-enzymatic system: effect on biocatalyst stability.  |  Van Hecke, W., et al. 2009. Biotechnol Bioeng. 102: 122-31. PMID: 18698649
    2. New spectrophotometric method for determining nitrogen dioxide in air using 2,2-azino-bis(3-ethyl benzothiazoline)-6-sulfonic acid-diammonium salt and passive sampling.  |  Salem, AA., et al. 2011. Anal Chem Insights. 6: 37-44. PMID: 21760708
    3. Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose.  |  Zhang, W., et al. 2014. Talanta. 120: 362-7. PMID: 24468383
    4. Sensitive and selective colorimetric detection of glutathione in human plasma with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and Ag+ ion.  |  Li, Y., et al. 2017. Spectrochim Acta A Mol Biomol Spectrosc. 173: 880-885. PMID: 27816888
    5. Colorimetric assay for monoamine oxidase in tissues using peroxidase and 2,2′-azinodi(3-ethylbenzthiazoline-6-sulfonic acid) as chromogen.  |  Szutowicz, A., et al. 1984. Anal Biochem. 138: 86-94. PMID: 6329037
    6. Assessment of substrates for horseradish peroxidase in enzyme immunoassay.  |  Al-Kaissi, E. and Mostratos, A. 1983. J Immunol Methods. 58: 127-32. PMID: 6339632
    7. Enzyme immunoassay ELISA and EMIT.  |  Engvall, E. 1980. Methods Enzymol. 70: 419-39. PMID: 6999296