EvaGreen® Dye is a patented green fluorescent nucleic acid dye that is essentially non-fluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. It is ideal for a wide variety of applications including qPCR and DNA melt curve analysis, HRM®, LAMP, digital PCR, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), capillary gel electrophoresis, and more. The dye is provided at 20X (25 uM) in water.
- Brighter and less inhibitory than SYBR® Green
- Environmentally safe, non-mutagenic, and non-cytotoxic
- Unsurpassed thermal stability, chemical stability, and photostability
- Excellent compatibility with multiplex PCR
- Directly visualize amplification products in a gel
- Compatible with common qPCR instruments and enzyme systems
The Unique Properties of EvaGreen® Dye
EvaGreen® Dye was designed with a novel “release-on-demand” mechanism. In the absence of DNA, the dye assumes a conformation that is inactive. When DNA is available, the dye shifts to an active conformation that binds DNA to emit fluorescence. This property of the dye provides a unique mechanism to continuously supply the active form of the dye from an inactive reserve, as more DNA is produced during the PCR process. As a result, EvaGreen® Dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for digital PCR, real-time qPCR, HRM®, and multiplex qPCR by DNA melt curve analysis. This technology also makes it possible to significantly shorten the chain extension time, enabling the use of fast PCR protocols.
PRODUCT ATTRIBUTES
| Detection method/readout | PCR/qPCR, HRM® |
|---|---|
| Excitation/Emission | 500/530 nm (with DNA) |
| Concentration | 20X (25 uM) |
| Storage Conditions | Store at -10 to -35 °C |