Human Bone Marrow cDNA (primed with random hexamer primers) - 30 reactions (30 PCR amplifications)

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553.00 €

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    Product Description:

    Human Bone Marrow full length tissue cDNA is made of RNA extracted from freshly harvested tissues of single healthy normal adult donor using classical guanidine isothiocyanate phenol: chloroform extraction method. RNA is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with Random Hexamer primer.

    Quality Control:
    The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
    The synthesized cDNA is also tested as template for PCR amplification of ß-actin gene. PCR product of ß-actin was visualized on 1% agarose gel.

    Applications:
    cDNA is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.

    Packing/shipping:
    Each cDNA sample is routinely shipped on dry ice in 1.5 ml vials (30ul each) and is enough for 30 reactions (30 PCR amplifications).