Human Insulin (INS) ELISA Kit - 96-wells plate

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    Uniprot No.: P01308

    Species: Homo sapiens (Human)

    Sample Types: serum, plasma, cell culture supernates, tissue homogenates

    Detection Range: 10 μIU/ml-160 μIU/ml

    Sensitivity: 2 μIU/ml

    Assay Time: 1-5h

    Sample Volume: 50-100ul

    Detection Wavelength: 450 nm

    Assay Principle: quantitative

    Measurement: Sandwich


    Materials provided:

      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be dismountable.
      • Six bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 6 ml--- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away the unbound solution and non-specific substances.
      • Substrate A 1 x 7 ml --- Contain oxidant.
      • Substrate B 1 x 7 ml--- Contain TMB. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Adhesive Strip (For 96 wells) 4 --- Cover the microtiter plate when incubation.
      • An instruction manual


    Materials not provided:

      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution


    User manual (sample only):