Recombinant Angola marburgvirus Glycoprotein minus the Transmembrane Region (MARV-Angola rGPΔTM) - 100ug
Description: Mature, recombinant, His-tagged Angola marburgvirus Glycoprotein minus the transmembrane domain (MARV-Angola rGPΔTM) is supplied as purified protein. MARVAngola rGPΔTM is produced in Sf9 insect cells using baculovirus for expression and is purified by FPLC.
Storage: 2-3 weeks at -20◦ C, -80◦ C long term Size: 100 µg of protein supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 0.334 mg/mL.
The theoretical molecular weight of the protein is ~60 kDa including the His-tag, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
Relevance: Recombinant glycoprotein provides a means for
antibody development, control protein for testing, and a tool to
Western Blot: Quality control testing demonstrates strong
detection of GP null and GP2 under reduced conditions.