bEnd.3 (BEND3) cells (endothelial cells isolated from brain tissue derived from a mouse with endothelioma) - 1 vial

Disease:

Endothelioma

Origin:

The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.

Species:

Mus musculus, mouse

Tissue:

Brain, cerebral cortex

Morphology:

Endothelial, endothelialpolyoma middle T antigen transformed

Properties:

Adherent

Strain:

BALB/c, 6 weeks

Medium:

DMEM (0683-C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:6 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

Size:

1 vial of ~1x10^6 cells (Lot dependent)

References for bEnd.3 cells:

1. Williams RL, Courtneidge SA, Wagner EF. Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene. Cell. 1988 Jan 15;52(1):121-131. PMID: 3345558