GFP Reporter Stable Cell Line HEK293 - 1 vial

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    Growth Properties: Adherent

    Host Cell: Human embryonic kidney cell line HEK293

    Morphology: Epithelial


    Shipping: Dry ice


    Cell Line Description:

    HEK293/GFP is derived from HEK293 cell line which has been transduced by

    lentiviruses containing EGFP under the control of CMV promoter, followed by

    puromycin selection of stable cells. This cell line is an ideal tool to support research in fluorescent tracing of HEK293 cells.


    Background:

    The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.


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