Human Oxidized Low Density Lipoprotein antibody IgM (OxLDL-IgM) ELISA Kit - 96-wells plate

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516.00 €

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    Assay range: 1.5 μg/L - 80 μg/L

    Sensitivity: 0.5 μg/L

    Standard:90 μg/L

     

    Principle:

    This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to OxLDL-IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for OxLDL-IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain OxLDL-IgM and HRP conjugated OxLDL antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of OxLDL-IgM. You can calculate the concentration of OxLDL-IgM in the samples by comparing the OD of the samples to the standard curve.


    User manual: