Ar.Qual Agrobacterium rhizogenes Chemically Competent Cells - 10x 100ul
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    Product Overview:

    The genotype of Ar.Qual Chemically Competent Cell is Agrobacterium rhizogenes (str R,Cam R) Ar Qual Ri (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The Ar.Qual Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid with a broad host range (corn, tobacco, tomato, citrus, etc.) and streptomycin and chloramphenicol resistance.


    Ar.Qual Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.


    1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.

    2. Do not pipette or vortex cells.

    3. Plating volume can be adjusted accordingly.

    4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.


    Store at - 80 °C for 12 months.Kit ComponentsAr.Qual: 100 μL/tube * 10 tube/50 tube/100 tube.

    Transformation efficiency:

    Transformation efficiency of Ar.Qual Chemically Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.