Overview: Goat polyclonal antibody to mCerulean (Cerulean fluorescent protein). mCerulean is a basic (constitutively fluorescent), monomeric engineered derivate of green fluorescent protein (GFP) isolated from Aequorea victoria. mCerulean is a ~27 kDa protein that is optimally excited at a 433 nm and has a maximum of emission at 575 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Other Names: green fluorescent protein antibody.
Other Names: green fluorescent protein antibody.
Concentration: 3 mg/ml
Antibody Type: Primary
Target Type: anti-mCerulean
Accession Number: N/A
Host Species: Goat
Immunogen Species: N/A
Immunogen Type: Recombinant protein
Immunogen: Purified recombinant fluorescent protein produced in E. coli.
Isotype: IgG
Clonality: Polyclonal
Confirmed Reactive Species: Reacts against Green Fluorescent Protein
Specificity Detail: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).
Buffer: PBS, 20% glycerol and 0.05% sodium azide
Purification: Epitope affinity purified
Conjugation: Unconjugated
Format: N/A
Application: WB, IF, IHC-P, IHC-F, IEM
Concentration/Dilution: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500